Reporter

Part:BBa_K1641032:Design

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-12)


Reporter of Invertase activity of Vcre, pInv-rep-101VloxPM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All reporter is constructed as following pattern: Constructive promoter :: RTS :: up-side-down RBS-mcherry-(ssra) :: RTS :: terminator


Source

This brick is constructed through cut-and-link of other bricks, without risky sources.

References