Translational_Unit
Part:BBa_K1641005:Design
Designed by: Pai Li Group: iGEM15_SYSU_CHINA (2015-09-09)
Fusion protein of Flp-EGFP-ssra, with RBS at beginning
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 522
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Ssra-Tag is efficiently cleaning the protein when not under induction, and helps to reduce leakage expression. C-term EGFP fusion on Flp seems have far better activity than N-term fusion.
Source
Flp is from BBa_K1015001 and EGFP::ssra-tag from pBI121-EGFP in Yongjun Lu's lab is fused by overlap-PCR; others through connection of bricks.