Translational_Unit

Part:BBa_K1641005:Design

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-09)


Fusion protein of Flp-EGFP-ssra, with RBS at beginning


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 522
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ssra-Tag is efficiently cleaning the protein when not under induction, and helps to reduce leakage expression. C-term EGFP fusion on Flp seems have far better activity than N-term fusion.


Source

Flp is from BBa_K1015001 and EGFP::ssra-tag from pBI121-EGFP in Yongjun Lu's lab is fused by overlap-PCR; others through connection of bricks.

References