Composite

Part:BBa_K1638036:Design

Designed by: Jens Sivk�r Pettersen   Group: iGEM15_SDU-Denmark   (2015-09-14)


Sce VMA Intein with promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 897
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 563
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1440
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Intein sequence: pTYB21 (IMPACT kit)

References

[1]: IMPACT™ Kit. 2015. New England Biolabs; [accessed 2015 Sep 11]. https://www.neb.com/products/e6901-impact-kit