Part:BBa_K1632033

fim switch[default OFF](Tokyo_Tech/J23119)_rbs_gfp_rbs_lasI

The fim switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the fim switch by adding the Fim recombinase.
We designed this fim switch which has a J23119 promoter. Also between the promoter and the inverting site, there are two restriction enzyme sites in each front (SalIand BamHI) and back (BglII and MluI)(Fig. 1. Tokyo Tech fim switch (Tokyo_Tech/J23119) design). So the promoter can easily be replaced. Except for insertion of restriction enzyme cut sites, basically, the design of fim switch(Tokyo_Tech) is similar with fim switch(wild-type).

Fig. 1. Tokyo Tech fim switch (Tokyo_Tech/J23119) design (Up:on state Down:off state)

When the state of fim switch is [ON], GFP and 3OC12HSL are expressed. But when the state of fim switch is [OFF], GFP and 3OC12HSL are not expressed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 98
  Illegal NheI site found at 121
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 92
  Illegal BglII site found at 1865
  Illegal BamHI site found at 133
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1427
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1102