Composite

Part:BBa_K1632018:Design

Designed by: Jun kawamura   Group: iGEM15_Tokyo_Tech   (2015-08-30)


J23100_rbs_lasR_TT_Plux_rbs_fimE(wild-type)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
    Illegal AgeI site found at 1034
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 927


Design Notes

sequence confirmed

Materials and Methods

Source

Composite of BBa_K553003, BBa_K1632015

References