Composite

Part:BBa_K1631010:Experience

Designed by: Yuto Yamanaka   Group: iGEM15_UT-Tokyo   (2015-09-14)


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Applications of BBa_K1631010

We tried to confirm the quasi-lysis, but regardless of induction with 1mM IPTG, OD600 is decreased after about 4 hours. This maybe caused by the leak from lac promoter(BBa_R0011). It is considered that quasi-lysis is occuered at low expression level of Colicin Lysis Protein, and even if the expression level is bigger than that point, the effectiveness to E. coli is not so different.


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User Reviews

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