Coding

Part:BBa_K1628101

Designed by: Xinhao Song   Group: iGEM15_Nankai   (2015-08-04)

pgsB

pgsB is a gene responsible for γ-PGA synthesis in pgsBCA operon. Protein PgsBCA is a membrane protein and subunit PgsB’s main function is gathering substrate glutamic acid for γ-PGA synthesis (showed in Figure 1).

PgsBCA1 NK.png

Funtion of part pgsB and pgsCA were validated through fermentation experiment. Wild type Bacillus amyloliquefaciens NK-1 strain could produce 3-4g/L γ-PGA after 48 hours of fermentation. In previous work in our laboratory, we also tested heterologous expression of γ-PGA in E. coli. We constructed different expression vectors containing pgsBCA genes and transformed them separately into E.coli JM109. The average production of γ-PGA in E. coli JM109 is around 0.5g/L.

PgsBCA2 NK.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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