Coding

Part:BBa_K1621006:Design

Designed by: Ramona Emig, Rabea Jesser, Julika Neumann, Lara Stuehn   Group: iGEM15_Freiburg   (2015-08-28)


Dihydroxyacid dehydratase (Salmonella Typhimurium)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Two recognition sites for PstI have been removed by site-directed mutagenesis. Therefore, the guanine residues at positions 96 and 679 have been mutated into cytosines. This results in one silent mutation and an amino acid exchange from alanine to proline at position 227 of the primary structure.


Source

The part is derived from Salmonella typhimurium. The sequence as well an expression plasmid carrying the part in a His-tagged variant were obtained from Meyer et al. (2012).

References