Coding
SmtA

Part:BBa_K1620007:Design

Designed by: Celio Dias Santos Jr   Group: iGEM15_UFSCar-Brasil   (2015-09-04)


Synechococcus metallothionenin A (SmtA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 121
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to eliminate an internal restriction site for PstI, the 98th base was changed G -> A. No residues were changed.

Source

This part is an improvement of part BBa_K519010. It was synthesized by IDT as G-block and amplified by PCR using the following primers:

Fwd: 5' - GAA TTC GCG GCC GCT TCT AGA GAT GAC CTC AAC AAC GTT GGT - 3'

Rev: 5' - TAC TAG TAG CGG CCG CTG CAG TTA GCC GTG GCA GTT ACA GC - 3'

After this, the 3A assembly method was carried out.

References