Coding
Part:BBa_K1616022:Design
Designed by: Johanna Chesnel Group: iGEM15_IONIS_Paris (2015-09-18)
pDawn - Endolysin/Holin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1405
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2755
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2689
Illegal NgoMIV site found at 2787
Illegal NgoMIV site found at 3670
Illegal NgoMIV site found at 3742
Illegal NgoMIV site found at 3832
Illegal NgoMIV site found at 3850
Illegal NgoMIV site found at 4344
Illegal AgeI site found at 306
Illegal AgeI site found at 376
Illegal AgeI site found at 3384
Illegal AgeI site found at 4512 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4401
Illegal BsaI.rc site found at 3283
Design Notes
The original sequence of pDawn have some illegal sites that have been removed.
Source
This part is composed of Holin/Endolysin toxin BBa_K1616005 and pDawn system BBa_K1616019