Coding

Part:BBa_K1616003:Design

Designed by: Johanna Chesnel   Group: iGEM15_IONIS_Paris   (2015-09-17)


VVD link to YC155 (YFP Cter split)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of VVD had 2 illegal sites PstI; that have been removed.


Source

Assembly the sequence of photoreceptor VVD (without illegal site), a linker(1) and then the C terminal of YFP split(1)

(1) Tom Kerppola, Ph. D, investigator at the Howard Hughes Medical Institute as well as Professor in the University of Michigan

References