Coding
CBDcipA

Part:BBa_K1615111:Design

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-08-27)

CBDcipA with N and C-terminal linker in RFC25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In an effort to improve the CBDCipA existing in the registry from iGEM14_Imperial we used the sequence from BBa_K1321014 and made it RFC25 compatible by removing the illegal EcoRI site at 148.


Source

CBDcipA from Clostridium thermocellum

References