Coding
mRFP

Part:BBa_K1615089:Design

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-08-31)

mRFP RFC25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E. coli and RFC 25 compatible. There is an existing RFC25 compatible RFP (BBa_K1351021) in the registry from iGEM14_LMU-Munich which was the basis for our sequence. We then added the RFC25 prefix without the Shine-Dalgarno sequence.


Source

mRFP highly engineered variant originally from Discosoma striata.

References