DNA

Part:BBa_K1614008

Designed by: Daniel Heid   Group: iGEM15_Heidelberg   (2015-09-16)

His Tag AptaBody (A5)

Notice: Functional DNA

This part is a sequence of a functional ssDNA. It is only active as single-stranded DNA. It can not be cloned into a plasmid. For use order it as a DNA oligo.


His Tag AptaBody is a sequence consisting of a His-Tag aptamer and the HRP-mimicking DNAzyme for use in Western Blot applications. Between the His Tag aptamer and the HRP mimicking DNAzyme a 5x poly-A spacer is placed ensuring the functionality for ligand binding and peroxidase activity.

Optimal peroxidase activity is reached with hemin concentration equimolar ro HRP-concentration, at pH 8.5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None