Device

Part:BBa_K1613007:Design

Designed by: Ko-yu Chang   Group: iGEM15_HSNU-TAIPEI   (2015-09-05)


A composite part that can detect some toxins.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 64
    Illegal suffix found in sequence at 1653
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 64
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 1654
    Illegal PstI site found at 1668
    Illegal NotI site found at 70
    Illegal NotI site found at 1661
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 64
    Illegal BglII site found at 1483
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 64
    Illegal suffix found in sequence at 1654
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 64
    Illegal XbaI site found at 79
    Illegal SpeI site found at 1654
    Illegal PstI site found at 1668
    Illegal NgoMIV site found at 403
    Illegal NgoMIV site found at 490
    Illegal AgeI site found at 473
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1203


Design Notes

use cyp1a2 to detect some toxins like aflatoxin B1


Source

We use humen liver gene cyp1a2(genebank:NM_000761),but we do some chang to remove the restriction site.

References