Coding

Part:BBa_K1613004:Design

Designed by: Ko-yu Chang   Group: iGEM15_HSNU-TAIPEI   (2015-09-05)


To detect toxicant harmful to human liver


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1400
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 320
    Illegal NgoMIV site found at 407
    Illegal AgeI site found at 390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1120


Design Notes

The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP1A2 is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include aflatoxin B1, and acetaminophen.


Source

We use humen liver gene cyp1a2(genebank:NM_000761),but we do some chang to remove the restriction site.

References