Part:BBa_K1610201:Design

human Antichymotrypsin with mutations for 3 amino acid changes (ACT3m)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 579
  Illegal XhoI site found at 954
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 880
  

Design Notes

There were originally two PstI cutting sites that have been mutated off (silent mutations).

Source

This part was derived off of Bba_K1610200, a cDNA clone provided by Sino Biological. The mutations on the sequence are later made by Mission Biotech.