Coding

Part:BBa_K1608000:Design

Designed by: Ching-Yuan Chen, Yi-Ru Liu, Pei-Hong Chen   Group: iGEM15_Mingdao   (2015-09-10)

SR/pSB1C3 (Fire Retardant BioBrick Part)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 541
    Illegal BglII site found at 610
    Illegal BglII site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • No EcoRI, XbaI, SpeI, PstI sites on the gene. It is easy to be used as a standard BioBrick part.


We designed two primers to amplify the SR genes by PCR, followed by XbaI and PstI digestion and ligated to pSB1C3. The plasmid DNA has been checked by colony PCR, restriction enzyme check and sequencing confirmation.


Primers:

  • 1.SR-XbaI-F: 5'- AAAAGGTCTAGATGTCGGGAGGTGGTGTGATTCGTG -3'
  • 2.SR-SpeI-PstI-R: 5'-AAGAAACTGCAGCGGCCGCTACTAGTATTATGTACGAGAGCGAGA -3'


PCR product size: ~785 bp


Plasmid map:

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For more DNA gel data & sequencing data, please go to our [http://2015.igem.org/Team:Mingdao/Parts page]

Source

The gene source is cloned from cDNA of Homo sapiens.

References

A human importin-beta family protein, transportin-SR2, interacts with the phosphorylated RS domain of SR proteins. [http://www.ncbi.nlm.nih.gov/pubmed/10713112 J Biol Chem. 2000]