Composite

Part:BBa_K1605005:Design

Designed by: Mike Toomey   Group: iGEM15_WashU_StLouis   (2015-09-14)

Ptet mRFP nifE RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 638
    Illegal AgeI site found at 750
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Had to take into consideration desired translation initiation rate


Source

Salis Lab RBS calculator

References

A. Espah Borujeni, A.S. Channarasappa, and H.M. Salis, "Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites", Nucleic Acid Research, 2013 H.M. Salis, E.A. Mirsky, C.A. Voigt, Nat. Biotech., 2009