Part:BBa_K1597003:Design

bslA, forms hydrophobic surface layer on biofilm

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We had to change nucleotide 387 from a cytosine into a thymine in order to get rid of an EcoRI restriction site but not change the amino acid sequence

Source

Genomic DNA from Bacillus subtilis 168