Coding

Part:BBa_K1595024

Designed by: Jonlin Chen   Group: iGEM15_Cornell   (2015-09-13)

MBP with EcnB from Mannheimia haemolytica

T7 Promoter + Ribosome binding site + MBP + TEV + EcnB + 6x Hist tag + terminator. Coding device for anti-microbial peptide derived from Mannheimia haemolytica EcnB with a stabilization maltose-binding protein and TEV cutsite.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 138


[edit]
Categories
Parameters
None