Coding
Part:BBa_K1595004:Design
Designed by: Jonlin Chen Group: iGEM15_Cornell (2015-09-05)
EcnB from Bordetella avium
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 129
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 129
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 129
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 129
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Use in conjunction with strain-expressing T7 polymerase
Internal cutsite for PstI
Source
Synthesized by Integrated DNA Technologies
References
NCBI Protein Database: WP_012417955