Part:BBa_K1593207
micF Promoter, Sensing Antibiotics
This is a promoter sensing sulfoamide which is previously selected from by Sahar Melamed and Shimshon Belkin
Usage and Biology
Description
micF, an antisense ncRNA targeting ompF RNA, main endogenous porin of Escherichia coli, participating in post-transcriptionally regulation of this outer membrane protein F(ompF). micF is also regarded as one of the promoter of soxS-regulated gene.
micF was originally tested by Sahar Melamed and Shimshon Belkin to research the antibiotic resistance of bacteria and features of selected sensing promoters. Sensitivity and specificity of micF was presented, showing its relatively specific signal response when treated with:
- sulfamethoxazole-255.2;
- sulfadimethoxine-44.9;
- colistin-36.9.
The maximal induction of micF strains tested in the course of 10h of exposure.
Application
In accordance of micF being responsive to the target compound, we implement micF to sense the existence of sulfamethoxazole and sulfadimethoxine in watersample.
Experimental Result
micF and soxS, these two antibiotic substance responding promoters were extracted from E. coli Top 10(K-12 strain) by conducting polymerase chain reaction on its genome.
Reference
A bacterial reporter panel for the detection and classification of antibiotic substances Microbial Biotechnology (2012) 5(4), 536–548 Sahar Melamed, Shimshon Belkin
MicF: An Antisense RNA Gene Involved in Response of Escherichia coli to Global Stress Factors 2001 JMB doi:10.1006/jmbi.2001.5029
Sequence and Features
| None |