Composite
Part:BBa_K1592018:Design
Designed by: Shuyan Tang Group: iGEM15_HUST-China (2015-09-09)
Pgal1+rox1+cyc1_terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1012
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 84
Illegal AgeI site found at 1224 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1005
Illegal BsaI.rc site found at 1055
Design Notes
none
Source
The information of basic parts sequence is from iGEM registry and the sequences are synthesized by IDT. We assemble them by 3A assembly method.