Part:BBa_K1592006:Design

GalAD-CIB1 Fusion for Yeast-Two-Hybrid

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 445
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 407
  Illegal XhoI site found at 1474
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 568
  Illegal AgeI site found at 1461
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

No

Source

Part cloned from pRMH120 of Addgene.

References

Hughes R M, Bolger S, Tapadia H, et al. Light-mediated control of DNA transcription in yeast[J]. Methods, 2012, 58(4): 385-391.