DNA

Part:BBa_K1588003:Design

Designed by: Juan David Sifontes   Group: iGEM15_UNA_Honduras   (2015-09-18)


tetravalen protein with epitopes from dengue virus to generate immune response


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1195
    Illegal PstI site found at 1348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1195
    Illegal PstI site found at 1348
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1195
    Illegal PstI site found at 1348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1195
    Illegal PstI site found at 1348
    Illegal NgoMIV site found at 796
    Illegal NgoMIV site found at 1606
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 875


Design Notes

1. is only one polypeptide chain 2. bacteria cells export them 3. low molecular weight 4. big exposed loops where we could insert antigenic determinants (epitopes)



Source

from Vibrio cholerae gene for Chitin Oligosaccharide Binding Protein

References