Device

Part:BBa_K1587007:Design

Designed by: Alexandre Le Scornet   Group: iGEM15_Toulouse   (2015-09-15)


Formate production


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 405
    Illegal BamHI site found at 2807
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 514
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2995


Design Notes

Source

Both genes are from E. coli :
- pflB http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=951272&to=953554&strand=true

- pflA http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=950340&to=951080&strand=true

The strong RBS and terminator were used according to respectively BBa_B0030 and BBa_B1006

References

- Crain AV, Broderick JB. 2014. Pyruvate Formate-lyase and Its Activation by Pyruvate Formate-lyase Activating Enzyme. J Biol Chem 289:5723–5729.

- Wei X-X, Zheng W-T, Hou X, Liang J, Li Z-J, Wei X-X, Zheng W-T, Hou X, Liang J & Li Z-J (2015) Metabolic Engineering of Escherichia coli for Poly(3-hydroxybutyrate) Production under Microaerobic Condition. BioMed Research International, BioMed Research International 2015, 2015: e789315