Reporter

Part:BBa_K1584035:Design

Designed by: Seung Meen Choi, Brittany Lee, Nu Ri Choi, SOOJI LEE, Hyun Min Park   Group: iGEM15_Elan_Vital_Korea   (2015-08-31)

Constitutive LuxR plus AHL-inducible LacZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 908


Design Notes

There are few design considerations concerning BBa_K1584035. While cutting the EcoRI and Xbal sites there is a chance that the promoter might be cut off. Therefore, one would need to be extra careful when cutting the sites. Also, after the LuxR a terminator is always required. If there is no terminator, then the LacZ would not function.


Source

J61100, C0062, K823017, R0062, I732006

References