Composite

Part:BBa_K1583205:Design

Designed by: Stefan Robert Marsden   Group: iGEM15_TU_Delft   (2015-09-04)


High constitutive promoter + CsgB & CsgC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We inserted non-coding scar sequences after the promoter and RBS to prevent missing initial codons during transcription/translation.


Source

The RBS and CsgC were synthesized from the genomic sequence of E.coli K-12 MG1655. The promoter is part of the constitutive promoter family made by John Anderson from iGEM Berkeley 2006.Non-coding scar sites were designed at random.

References