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Coding

Part:BBa_K1583112:Design

Designed by: Stefan Robert Marsden, Max van 't Hof, Samantha Basalo Vazquez   Group: iGEM15_TU_Delft   (2015-09-15)

pRha + CsgA & GFP in same operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1308

Design Notes

To measure the transcriptional and translational rates of CsgA production, we created this device. The beginning of the device consists of our standard parts: the rhamnose promoter and the CsgA gene.
However, there is no terminator located upstream the gene. In this manner we were able to clone the gene from the biobrick BBa_I13504 coding for GFP behind CsgA and into the same operon.

Source

This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft team and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from E. coli K-12 MG1655.