Coding

Part:BBa_K1583110:Design

Designed by: Stefan Robert Marsden   Group: iGEM15_TU_Delft   (2015-09-04)


pRha + Mfp5_CsgA_His fusion protein & pTET + RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1168
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 635
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RFP operon was placed in front of the coding operon to take advantage of its double terminators.


Source

The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).

References