Part:BBa_K1583108:Design

pRha + CsgA with Hydroxyapatite-affinity tag & pTet + RFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 635
  Illegal AgeI site found at 747
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

For our design, we took advantage of the double terminator present in the biobrick I13521 by placing it in front of our genes.

Source

The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).