Coding
Part:BBa_K1583104:Design
Designed by: Stefan Robert Marsden Group: iGEM15_TU_Delft (2015-09-04)
pRha + Mfp5_CsgA_His fusion protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 245
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A mutation had to be introduced at base pair 766 to remove a PstI restriction site. The protein sequence was not changed in doing so.
Source
This part originates from the E.coli K-12 MG1655 genome and from mussels. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1]
1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.