Scar
Part:BBa_K1583054:Design
Designed by: Stefan Robert Marsden Group: iGEM15_TU_Delft (2015-09-04)
Non-coding scar site in between RBS and gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We inserted non-coding scar sequences after the promoter and RBS to prevent missing initial codons during transcription/translation.
Source
The RBS and CsgC were synthesized from the genomic sequence of E.coli K-12 MG1655. The promoter is part of the constitutive promoter family made by John Anderson from iGEM Berkeley 2006.