Composite

Part:BBa_K1582019:Design

Designed by: Dongqi Bao   Group: iGEM15_Tianjin   (2015-09-05)

LC+inJanus-m Fusion Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 600
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1075


Design Notes

Linker:
GGTGGTGGTGGTTCTGGTGGTGGTGGTTCT
Codon optimized to:
ACCTACTCTGACAACCGTTACGGTGGTACCTGCGACCCGGACGGTTGCGACTGGAACCCGTACCGTCTGGGTAAC

Source

References