Protein_Domain
Tm-Fluo-li

Part:BBa_K157014

Designed by: iGEM Team Freiburg 2008   Group: iGEM08_Freiburg   (2008-10-26)

Tm-Fluo-linker-cYFP

C-terminal part of the Split fluorophore YFP fused to the trans-membrane region of the Erbb1/EGF-receptor with the fluolinker in between.
Part was derived by cloning of BBa_K157002, BBa_K157009 and BBa_K157007.
For further information see the description of the parts mentioned above.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 385
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter/fluorescence
//proteindomain/internal
Parameters
None