Generator

Part:BBa_K156021

Designed by: George McArthur IV   Group: iGEM08_Virginia   (2008-10-28)


Promoter + RBS + phaA + double terminator

This device is a synthetic gene that generates PhaA, an enzyme that catalyzes the synthesis of acetoacetyl-coenzyme A from two molecules of acetyl-coenzyme A. The enzyme can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 967
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 933
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 306
    Illegal NgoMIV site found at 318
    Illegal AgeI site found at 681
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/biosynthesis/plastic
Parameters
None