DNA

Part:BBa_K1560003:Design

Designed by: Chris Lee, Charles Paine, Max Genetti, Jeff Nivala, David Bernick   Group: iGEM14_UCSC   (2014-10-01)


Knock-In Knock-Out Vector for HVO_1373


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 737
    Illegal NgoMIV site found at 947
    Illegal NgoMIV site found at 1496
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 1912
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 111
    Illegal BsaI site found at 249
    Illegal BsaI site found at 1019


Design Notes

Construct assembled using the Gibson method. Therefore Gibson tagged primers were designed for each sub part.


Source

H. volcanii genomic sequence

References