DNA

Part:BBa_K1560002

Designed by: Chris Lee, Charles Paine, Max Genetti, Jeff Nivala, David Bernick   Group: iGEM14_UCSC   (2014-09-29)

Knock-In Knock-Out Vector for HVO_1199

This part is a non-replicating plasmid designed to knock out the Acd3 gene in Haloferax Volcanii as described here (Allers et al., 2004). Thus this part contains both upstream and downstream homologous regions to Haloferax Volcanii necessary to knockout the gene as described in the paper above. Between the flanking sequences are 40 base pairs of non-sense DNA that will replace the wild type Acd3 in the Haloferax Volcanii genome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1605
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1521
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 737
    Illegal NgoMIV site found at 947
    Illegal NgoMIV site found at 1496
    Illegal NgoMIV site found at 1875
    Illegal NgoMIV site found at 2195
    Illegal NgoMIV site found at 2391
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 111
    Illegal BsaI site found at 249
    Illegal BsaI site found at 1019
    Illegal BsaI site found at 1860


[edit]
Categories
Parameters
None