Part:BBa_K1559010:Design

RFP-Targeting protospacer for CRISPR/Cas9 system

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Source

The protospacer + PAM in Part:BBa_J04450 was found by using the DNA 2.0 CRISPR gRNA design tool:

https://www.dna20.com/eCommerce/cas9/input

Settings:

• Species: E. coli K12 MG1655
• PAM: NGG
• Choose Wild-type Cas9 instead of Nickase
• gRNA offset: use default settings
• Target a sequence: use sequence of Part:BBa_J04450