Measurement
Pvgb+GFP
Part:BBa_K1555003:Design
Designed by: Li Huiyu, Jiang Zhenxiong, Wang Kun Group: iGEM14_NJAU_China (2014-10-06)
GFP induced by micro-aerobic promoter vgb
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 808
Design Notes
We used restriction sites SpeI and PstI of BBa_K561000, and restriction sites XbaI and PstI of BBa_E0840 to restrict the plasmid and successfully construct this measurement device. Our linked part did have capability to measure the expression of promoter vgb.
Source
All subparts are obtained in 2014 Distribution Kits. All of them are designed and assembled by students.