Measurement
Pvgb+GFP

Part:BBa_K1555003:Design

Designed by: Li Huiyu, Jiang Zhenxiong, Wang Kun   Group: iGEM14_NJAU_China   (2014-10-06)

GFP induced by micro-aerobic promoter vgb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 808


Design Notes

We used restriction sites SpeI and PstI of BBa_K561000, and restriction sites XbaI and PstI of BBa_E0840 to restrict the plasmid and successfully construct this measurement device. Our linked part did have capability to measure the expression of promoter vgb.


Source

All subparts are obtained in 2014 Distribution Kits. All of them are designed and assembled by students.

References