Composite

Part:BBa_K1550010:Design

Designed by: Jesse Goldenberg   Group: iGEM14_BUGSS_Baltimore   (2014-10-17)


BmtA (metallothionein) codon optimized for E. coli, with RBS and tt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 148
    Illegal AgeI site found at 163
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 113


Design Notes

When used in E. coli for the sequestration of lead or zinc ions in solution, the part may or may not work better in concert with a genomic ZntA knockout. We plan to characterize this combination in the near future.


Source

The sequences for pre-existing basic parts besides K1550004 (BBa_B0034 and BBa_B0015) were incorporated as found in the iGEM registry. The sequence used for the BmtA (metallothionein) was codon optimized for E. coli (using the browser-based software, Gene Design). The native sequence comes from Oscillatoria brevis, and was sourced from an entry in the NCBI database by Liu,T., Nakashima,S., Hirose,K., Uemura,Y., Shibasaka,M., Katsuhara,M. and Kasamo,K. (2003).

References