Plasmid
pSB_LIC

Part:BBa_K155000:Design

Designed by: Marc Slingerland, Nathan Puhl   Group: iGEM08_Lethbridge_CCS   (2008-10-25)

LIC plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1092


Design Notes

LIC overhangs used are based on Aslanidis & DeJong (2000). Central restriction site had to be compatible with the NTP requirements of the overhangs; chose EcoRV.


Source

From PCR amplification of pSB1A2 with overhang primers.

References

Aslanidis, C. & de Jong P.J. (1990) “Ligation-independent cloning of PCR products (LIC-PCR).” Nucl. Acids Res. 18:20, 6069-6074.