Project

Part:BBa_K153002:Design

Designed by: Dmitry O. Malyshev   Group: iGEM08_University_of_Sheffield   (2008-10-29)

CsrB with promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 49
    Illegal SpeI site found at 582
    Illegal PstI site found at 568
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 49
    Illegal SpeI site found at 582
    Illegal PstI site found at 568
    Illegal NotI site found at 573
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 49
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 49
    Illegal SpeI site found at 582
    Illegal PstI site found at 568
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 49
    Illegal SpeI site found at 582
    Illegal PstI site found at 568
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 498


Design Notes

In the natural E.Coli system CsrA is an important gene, the product of which inhibits many genes responsible for glycogen formation, gluconeogenesis, and biofilm formation. During a quorum sensing event, it is in turn inhibited by CsrB, thus allowing biofilm formation.

Source

to be added

References