Project
Part:BBa_K153001:Design
Designed by: Dmitry O. Malyshev Group: iGEM08_University_of_Sheffield (2008-10-29)
CsrA without promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 226
Illegal PstI site found at 212 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 226
Illegal PstI site found at 212
Illegal NotI site found at 217 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 70
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 226
Illegal PstI site found at 212 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 226
Illegal PstI site found at 212 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 179
Design Notes
In the natural E.Coli system CsrA is an important gene, the product of which inhibits many genes responsible for glycogen formation, gluconeogenesis, and biofilm formation. During a quorum sensing event, it is in turn inhibited by CsrB, thus allowing biofilm formation.
Source
to be added