Composite

Part:BBa_K1529150:Design

Designed by: Shoko Suzuki   Group: iGEM14_Tokyo_Tech   (2014-09-15)


PT7_obcA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 276
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 276
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1420
    Illegal XhoI site found at 183
    Illegal XhoI site found at 1307
    Illegal XhoI site found at 1751
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 276
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 276
    Illegal NgoMIV site found at 268
    Illegal NgoMIV site found at 599
    Illegal NgoMIV site found at 627
    Illegal NgoMIV site found at 663
    Illegal NgoMIV site found at 893
    Illegal NgoMIV site found at 1670
    Illegal NgoMIV site found at 1721
    Illegal AgeI site found at 205
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1


Source

1

References

[1]Oh, Juntaek et al. “Structural basis for bacterial quorum sensing-mediated oxalogenesis.” The Journal of biological chemistry vol. 289,16 (2014): 11465-11475. doi:10.1074/jbc.M113.543462 [2]Nakata, Paul A, and Cixin He. “Oxalic acid biosynthesis is encoded by an operon in Burkholderia glumae.” FEMS microbiology letters vol. 304,2 (2010): 177-82. doi:10.1111/j.1574-6968.2010.01895.x