Part:BBa_K1523023

nahG coding sequence

salicylate hydroxylase from Pseudomonas putida

Salicylate + NADH + O2 = catechol + NAD+ + H2O + CO2.

It contains a FAD binding domain. FAD is the cofactor.

Isogenesis Structure

salicylate hydroxylase from Rhodococcus jostii

The Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 208
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 208
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 208
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 208
  Illegal NgoMIV site found at 120
  Illegal NgoMIV site found at 142
  Illegal NgoMIV site found at 1042
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1123