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This was a intermidiate caracterization construction designed to evaluate the LasR-QteE interaction. In this construction there is no QteE being produced, while there is constitutive LasR expression. Having said that, what is expected to be seen here is a GFP fluorescence due to the activation of the PlasR promoter by LasR protein.

Here we have the result from the Flow Cytometry analysis. The first graph is the result obtained for the E. coli control (no fluorescence). The second is the result for the KX construction. The area shown bellow is corresponds to the GFP fluorescence.

Although the signal is weak its was identified in triplicate. What might have happend is that the GFP output signal is not generated directly as a consequence of the LasR production. The LasI expression followed by the HSL production are essencial for the LasR to induce the GFP. This necessery steps may have weaken the output intensity.