RNA

Part:BBa_K152011:Design

Designed by: David Johnston Monje   Group: iGEM08_Guelph   (2008-10-30)


GFP RNAi construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 898
    Illegal XbaI site found at 907
    Illegal SpeI site found at 1038
    Illegal PstI site found at 1102
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 898
    Illegal SpeI site found at 1038
    Illegal PstI site found at 1102
    Illegal NotI site found at 1046
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 898
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 898
    Illegal XbaI site found at 907
    Illegal SpeI site found at 1038
    Illegal PstI site found at 1102
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 898
    Illegal XbaI site found at 907
    Illegal SpeI site found at 1038
    Illegal PstI site found at 1102
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 996
    Illegal BsaI.rc site found at 663
    Illegal BsaI.rc site found at 1780


Design Notes

This part MAY have been made incorrectly (the antisense fragment got inserted in the sense orientation by accident). Stay tuned or try it out yourself to see what's up.


Source

Mix of two biobricks

References