RNA

Part:BBa_K152010:Design

Designed by: David Johnston Monje   Group: iGEM08_Guelph   (2008-10-30)


TB1 RNAi construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 254
    Illegal XbaI site found at 263
    Illegal SpeI site found at 394
    Illegal PstI site found at 458
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 254
    Illegal SpeI site found at 394
    Illegal PstI site found at 458
    Illegal NotI site found at 402
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 254
    Illegal BamHI site found at 112
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 254
    Illegal XbaI site found at 263
    Illegal SpeI site found at 394
    Illegal PstI site found at 458
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 254
    Illegal XbaI site found at 263
    Illegal SpeI site found at 394
    Illegal PstI site found at 458
    Illegal NgoMIV site found at 66
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 181
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 352


Design Notes

This part MAY have been made incorrectly (the antisense fragment got inserted in the sense orientation by accident). Stay tuned or try it out yourself to see what's up.


Source

Mix of two biobricks

References